杨敏生,内蒙古多伦县人,民进会员,澳门永利yl6776教授,博士,博士生导师,河北省政府特殊津贴专家。兼任河北省林木种植资源与森林保护重点实验室副主任,中国林学会林木遗传育种分会常务委员,中国林学会杨树委员会委员,中国林学会树木生理生化专业委员会委员,河北省生物工程学会副理事长,河北省政协委员,中国民主促进会保定市委副主委。主要从事林木遗传育种教学、科研和推广工作,主持过国家自然科学基金、转基因生物新品种培育重大专项子课题、“863计划”子课题、国家林业局公益项目、农业部中德农业合作项目、教育部博士点基金、河北省自然科学基金等国家及省级科研课题多项。1项成果获国家技术发明二等奖,主持完成的2项科研成果分别获得教育部科技进步二等奖和河北省科技进步二等奖,主持及主研参加的6项科研成果获省部级科技进步三等奖。国内外学术刊物上发表学术论文200余篇,其中SCI收录论文30余篇;主编和参编学术专著6部;培育林木良种4个,获新品种保护2个,授权专利5项。
教育经历
1993/09-1996/07,北京林业大学,林木遗传育种专业,博士研究生,导师朱之悌院士
1986/09-1989/07,北京林业大学,林木遗传育种专业,硕士研究生,导师:朱之悌院士
1980/09-1984/07,北京澳门永利yl6776,林业专业,大学本科,导师:沈国舫院士
2006/09-2006/11, 德国洪堡大学,高级访问学者
2002/07-2002/12, 德国林木遗传育种研究所,高级访问学者
1998/10-1999/10, 德国林木遗传育种研究所及洪堡大学,访问学者
工作经历
1997/09至今 澳门永利yl6776教授(2001/09遴选为博士生导师)
1993/09-1997/08,澳门永利yl6776副教授
1989/09-1993/08,河北澳门永利yl6776讲师
1984/07-1989/08,河北澳门永利yl6776助教
主要承担课程
1.博士课程:《林木遗传育种专题》
2.硕士课程:《林木改良专题》、《林业研究法与试验设计》
3.本科课程:《林木育种学》、《林木遗传学》
研究方向和领域
1.转基因杨树育种
2.林木种质资源评价与利用
3.观赏林木新品种选育与示范推广
主要科研项目(2015-2019)
2018/01-2020/12,国家科技重大专项子课题,转抗虫基因杨树新品种培育,974万元,主持。
2017/01-2019/12,国家林业与草原局科技发展中心项目,东北地区杨树资源收集评价及品种DNA指纹图谱构建,40万元,主持。
2017/01-2019/12,国家林业与草原局科技发展中心项目,转双抗虫基因107杨环境释放和生产性试验安全性监测,15万元,主持。
2018/01-2021/12,河北省基础研究计划项目,利用基因编辑技术提高杨树抗虫性研究,25万元,主持。
2014/01-2017/12,国家自然科学基金项目,转抗虫基因杨树多基因互作及高效表达机制研究,80万元,主持。
2013/01-2017/12,“863”计划项目子课题,杨树抗虫转基因育种技术研究,170万元,主持。
2016/01-2017/12,原国家林业局科技发展中心项目,杨树等3种植物属保护品种资源保存及DNA指纹标签建立研究,20万元,主持。
2014/01-2016/12,原国家林业局科技发展中心项目,转抗虫基因741杨环境释放和生产性试验安全性监测,20万元,主持。
2011/01-2015/12,国家林业公益性行业重大科研专项,北方主要林木品种指纹库构建及分子鉴定技术研究,179万元,主持。
主要科研奖励
“北方部分林木种质资源遗传评价及利用关键技术”,2016年获河北省科技进步二等奖,排名第一
“杨树板材林培育及其优质环保加工关键技术”,2011年获教育部科技进步一等奖,排名第一
“转双抗虫基因741杨”,2004年获国家技术发明二等奖,排名第五
“国内外刺槐资源评价及引种应用研究”,2009年获河北省科技进步三等奖,排名第一
“转基因杨树生态抗虫效应及安全性评价”,2007年获河北省自然科学三等奖奖,排名第二
“转双抗虫基因741杨繁育及栽培综合配套技术”,2005年获河北省科技进步三等奖,排名第一
“白杨双交杂种新无性系抗旱性生理基础及苗期鉴定研究”,2000年获河北省科技进步三等奖,排名第一
主要科研论文、著作(2015-2019)
1. Xiaoyue Yu, Lihui Zuo, Dandan Lua, Bin Lu, Minsheng Yang(通讯作者), Jinmao Wang. Comparative analysis of chloroplast genomes of five Robinia species: Genome comparative and evolution analysis. Gene, 2019, 689: 141-151
2. Yu Pan, Yan Dong, Ruixue Wang, Minsheng Yang(通讯作者). Generation of a promising universal RNAi vector system to control plant pests. Acta Physiologiae Plantarum, 2019, 41:18, doi.org/10.1007/s11738-019-2810-4
3. Yongtan Li Jun Zhang, Longfei Li, Lijuan Gao, Jintao Xu and Minsheng Yang(通讯作者). Structural and comparative analysis of the complete chloroplast genome ofPyrus hopeiensis—“Wildplants with a tiny population”—and three otherPyrusspecies. International Journal of Molecular Sciences,2018, 19, 3262; doi:10.3390/ ijms19103262
4. Guiying Wang, Yan Dong, Xiaojie Liu, Guosheng Yao, Xiaoyue Yu and Minsheng Yang(通讯作者). The current status and development of insect-resistant genetically engineered poplar in China. Frontiers in Plant Science, 2018, 9:1048,doi: 10.3389/fpls.2018.01408
5. Zuo Lihui, Yang Runlei, Zhen Zhixian, Liu Junxia, Huang Lisha, Yang Minsheng (通讯作者). A 5-year field study showed no apparent effect of theBttransgenic 741 poplar on the arthropod community and soil bacterial diversity, Scientific Reports, 2018, 8: 1956,DOI:10.1038/s41598-018-20322-3
6. Ren Yachao, Zhang Jun, Wang Guiying, Liu Xiaojie, Li Li, Wang Jinmao, Yang Minsheng (通讯作者). The Relationship between insect resistance and tree age of transgenic triploidPopulus tomentosaplants , Frontiers in Plant Science, 2018, 9:53, doi: 10.3389/fpls.2018.00053
7. Panfei Chen, Lihui Zuo1, Xiaoyue Yu, Yan Dong, Shuang Zhang, Minsheng Yang(通讯作者). Response mechanism inPopulus × euramericanacv. ‘74/76’ revealed by RNA-seq under salt stress. Acta Physiologiae Plantarum, 2018, 40:96, doi.org/10.1007/s1173 8-018-2676-x
8. Ren Yachao, Zhang Jun, Liang Haiyong, Wang Jinmao, Yang Minsheng (通讯作者). Inheritance and expression stability of exogenous genes in insect-resistant transgenic poplar. Plant Cell Tiss Organ Cult, 2017, 130(3): 567-576
9. Zhang Shuang, Zuo Lihui, Zhang Jun, Chen Panfei, Wang Jinmao, Yang Minsheng (通讯作者). Transcriptome analysis ofUlmus pumila‘Jinye’ responses to different shading involved in chlorophyll metabolism. Tree Genetics & Genomes, 2017, DOI 10.1007/s11295-017-1139-7
10. Zuo Lihui , Shang Aiqin, Zhang Shuang, Yu Xiaoyue, Ren Yachao, Yang Minsheng (通讯作者). The first complete chloroplast genome sequences ofUlmusspecies by de novo sequencing: Genome comparative and taxonomic position analysis. PLoS One, 2017.01.01,12(2): e0171264- e0171264
11. Dong Y, Ren YC, Zhang J, Qiu T, Cui HL, Yang MS (通讯作者). Construction of a new type of multi-gene plant transformation vector and genetic transformation of tobacco. Biologia Plantarum, 2017, 61(1): 13-23
12. Qiu Tong, Dong Yan, Ren Yachao, Wang Jinmao, Yang Minsheng (通讯作者), Zhang Jun. Effects of the sequence and orientation of an expression cassette in tobacco transformed by dual Bt genes. Plasmid, 2017, 89: 1-8
13. Lihui Zuo, Jun Zhang, Wenlin Zhang, Ruixue Wang and Minsheng Yang(通讯作者). Stepwise regression analysis of the correlation between leaf traits and SSR markers ofMalus sieversii. Pak. J. Bot., 2017, 49(6): 2255-2262
14. Shengliang Yuan, Yan Dong, Na Zhang, Yachao Ren, Minsheng Yang, Baojia Gao. Construction of high-effciency transformation vector with multiple insect- resistant genes and expression in tobacco. Acta Physiol Plant, 2017, DOI 10.1007/s11738-016-2338-9
15. Liu Dongyan, Zhang Jun, Dong Yan, Zhang Xu, Yang Minsheng (通讯作者), Gao, Baojia. Genetic transformation and expression ofCry1Ac-Cry3A-NTHK1genes inPopulusíeuramericana"Neva'', Acta Physiologiae Plantarum, 2016, 38(7):1-11
16. Yiwen Zhang, Jun Zhang, Jinping Lan, Jinmao Wang, Junxia Liu, Minsheng Yang(通讯作者). Temporal and spatial changes in Bt toxin expression in Bt-transgenic poplar and insect resistance in field tests. Journal of forestry research, 2016, 27(6): 1249-1256
17. RL Yang, AX Wang, J Zhang, Y Dong, MS Yang(通讯作者)and JM Wang. Genetic transformation and expression of transgenic lines ofPopulus x euramericanawith insect-resistance and salt-tolerance genes. Genetics and Molecular Research, 2016, 15(2): gmr.15028635
18. LN Xu, Y Dong, J Zhang, RX Wang, HM Liu, Q Yangand MS Yang(通讯作者).Effect of dual Bt-expression transformation vectors on transgene expression in tobacco. Genetics and Molecular Research, 2016, 15(3): gmr.15038293
19. Dong Yan, Du Shasha, Zhang Jun, Yang Minsheng (通讯作者), Wang Jinmao. Differential expression of dual Bt genes in transgene poplar Juba (Populus deltoidescv. 'Juba') transformed by two different transformation vectors, Canadian Journal of Forest Research, 2015.1, 45(1): 60-67
20. Y Ren, Y Dong, J Zhang, T Qiu and M Yang (通讯作者). Genetic transformation and expression detection of tobacco by using a multi-gene plant transformation vector. The Journal of Animal & Plant Sciences, 2015, 25(3 Suppl.1), 13-21
21. L Zuo, M Yang (通讯作者), J Zhang and H Liang. SSR primer select and analysis in Malus Mill genetic relationship research. The Journal of Animal & Plant Sciences, 2015, 25(3 Suppl.1), 128-133
22. DJ Zhang, JX Liu, ZY Lu, CL Li, E Comadaand MS Yang (通讯作者).Impacts of transgenic poplar-cotton agro-ecosystems upon target pests and non-target insects under field conditions, Genet. Mol. Res., 2015, 14 (3): 8125-8136
23. DJ Zhang, ZY Lu, JX Liu, CL Li and MS Yang(通讯作者). Diversity of arthropod community in transgenic poplar-cotton ecosystems. Genet. Mol. Res., 2015, 14 (4): 15713-15729
24. Yuhan Sun, Yuyao Zhang, Cunquan Yuan, Qing Yang, Cui Long, Yun Li and Minsheng Yang(通讯作者). Assessment of genetic diversity and variation ofAcer monomaxseedlings after spaceflight. Pak. J. Bot., 2015, 47(1): 197-202
25. 杨敏生,谷俊涛,王进茂,张军等.刺槐资源评价及引种研究.中国林业出版社, 2017
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